Prostaglandin E2 ELISA Kit - Monoclonal 514010 from Cayman Chemical

Product Specs
ItemProstaglandin E2 ELISA Kit - Monoclonal
CompanyCayman Chemical
Price Pricing Info Supplier Page View Company Product Page
Catalog Number514010
Quantity96 Solid
ApplicationsELISA
Detection Range7.8-1,000 pg/ml
Sensitivity80% B/B0: 15 pg/ml
ClonalityMonoclonal
Research AreaCyclooxygenase Pathway, Immunology & Inflammation, Inflammatory Lipid Mediators, Lipid Biochemistry, Prostaglandins

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Cayman Chemical

Cayman Chemical Company 1180 East Ellsworth Road

Ann Arbor, Michigan 48108

United States

Phone: (800) 364-9897
(734) 971-3640

Company Profile

Website: http://www.caymanchem.com/

(1) OnabotulinumtoxinA effects on trigeminal nociceptorsCephalalgiaNovember 12, 2023Ashley A Moore, Mariana Nelson, Christopher Wickware, Shinbe Choi, Gene Moon, Emma Xiong, Lily Orta, Amy Brideau-Andersen, Mitchell F Brin, Ron S Broide, et al.PGE2 levels were measured using commercially available ELISA kits (Cayman Chemical Company, 589001 and 514010) according to manufacturer’s instructions. Immunocytochemistry Trafficking of TRP channels to the

(2) Glucocorticoids alleviate particulate matter-induced COX-2 expression and mitochondrial dysfunction through the Bcl-2/GR complex in A549 cellsScientific ReportsNovember 2, 2023Yeon-Ji Park, June Heo, Yonghyeon Kim, Hyeseong Cho, Myeongkuk Shim, Kyunghyun Im, Wonchung LimPM-treated A549 cells was measured using an ELISA kit according to the manufacturer’s instructions (514010, Cayman, USA). Statistical analysis Data were expressed as means ± SE, and group comparisons were

(3) Isolation and Differentiation of Neurons and Glial Cells from Olfactory Epithelium in Living SubjectsMolecular NeurobiologyAugust 1, 2023Paula Unzueta-Larrinaga, Rocío Barrena-Barbadillo, Inés Ibarra-Lecue, Igor Horrillo, Aitor Villate, Maria Recio, J. Javier Meana, Rebeca Diez-Alarcia, Oihane Mentxaka, Rafael Segarra, et al.BMS213-2, Invitrogen, Thermo Fisher Scientific, Inc.; and Prostaglandin E 2 ELISA Kit—monoclonal, cat. no. 514010, Cayman Chemical, Ann Arbor, MI, USA) in culture medium samples, according to the manufacturer’s instructions.

(4) MSC therapy ameliorates experimental gouty arthritis hinting an early COX-2 inductionFrontiers in ImmunologyJuly 18, 2023Juan Pablo Medina, Ismael Bermejo-Álvarez, Sandra Pérez-Baos, Rosa Yáñez, María Fernández-García, Damián García-Olmo, Aránzazu Mediero, Gabriel Herrero-Beaumont, Raquel Largo7.4, 1mM EDTA was added. After centrifugation, PGE2 concentration was measured employing ELISA kit (514010, Cayman Chemical, USA, MI). Histological evaluation. SM inflammation was evaluated in hematoxylin-eosin-stained

(5) Crosstalk between diacylglycerol kinase and protein kinase A in the regulation of airway smooth muscle cell proliferationRespiratory ResearchJune 10, 2023Miguel A. Hernandez-Lara, Santosh Kumar Yadav, Stanley Conaway, Sushrut D. Shah, Raymond B. Penn, Deepak A. Deshpandeproliferation assay kit was from Life Technologies (Grand Island, NY, USA). PGE2 competitive ELISA (# 514010) was from Cayman Chemicals (Ann Arbor, MI, USA). Cell culture Human ASM cells were isolated from de-identified

(6) Extracellular Vesicles from Candida haemulonii var. vulnera Modulate Macrophage Oxidative BurstJournal of FungiMay 12, 2023Bianca T. M. Oliveira, Thales M. H. Dourado, Patrick W. S. Santos, Tamires A. Bitencourt, Carlos R. Tirapelli, Arnaldo L. Colombo, Fausto Almeidaquantified using a specific enzyme immunoassay (EIA Kit) according to the manufacturer’s instructions (514010, Cayman). The results for PGE2 concentrations are expressed in pg/mg. 2.9. Malondialdehyde Concentration The

(7) Synergistic Combination of Citrus Flavanones as Strong Antioxidant and COX-Inhibitor AgentAntioxidants (Basel)April 21, 2023Antonella Smeriglio, Nunzio Iraci, Marcella Denaro, Giuseppina Mandalari, Salvatore Vincenzo Giofrè, Domenico Trombettaextracellular medium was quantified (pg/mL) using the Prostaglandin E2 ELISA Kit—Monoclonal (Item No. 514010, Cayman Chemical Company, Ann Arbor, MI, USA) in accordance with the manufacturer’s protocol, by using

(8) High mobility group box 1, ATP, lipid mediators, and tissue factor are elevated in COVID‐19 patients: HMGB1 as a biomarker of worst prognosisClinical and Translational ScienceApril 1, 2023Amanda Roberta Revoredo Vicentino, Vanderlei da Silva da Silva Fraga-Junior, Matheus Palazzo, Natalia Recardo Amorim Tasmo, Danielle A. S. Rodrigues, Shana Priscila Coutinho Barroso, Sâmila Natiane Ferreira, Anna Cristina Neves Neves‐Borges, Diego Allonso, Marcelo Rosado Fantappié, et al.enzyme‐linked immunosorbent assay (ELISA) kits, according to the manufacturer's instructions (PGE2 No. 514010, LTB4 No. 520111, and cys‐LTs No.501070; Cayman Chemical, Ann Arbor, MI). Western blot analysis of

(9) Stabilization of E-cadherin adhesions by COX-2/GSK3β signaling is a targetable pathway in metastatic breast cancerJCI insightMarch 22, 2023Balamurugan K, Poria DK, Sehareen SW, Krishnamurthy S, Tang W, McKennett L, Padmanaban V, Czarra K, Ewald AJ, Ueno NT, et al.blood plasma and tumor tissues was quantified using a commercially available kit (Cayman Chemical, 514010). As previously described (46), fresh tumor tissue was homogenized and lysed in buffer containing 50

(10) Role of Noradrenaline and Adrenoreceptors in Regulating Prostaglandin E2 Synthesis Cascade in Inflamed Endometrium of PigsInternational journal of molecular sciencesMarch 20, 2023Jana B, Całka J, Bulc M, Witek K.Procedure The PGE2 concentration in the incubation medium was measured using the ELISA kits (cat. no. 514010, Cayman Chemical Co.), following the manufacturer’s protocols. The standard curve for PGE2 ranged from

(11) Circumvention of luteolysis reveals parturition pathways in mice dependent upon innate type 2 immunityImmunityMarch 14, 2023Siewiera J, McIntyre TI, Cautivo KM, Mahiddine K, Rideaux D, Molofsky AB, Erlebacher A.516011 Prostaglandin D2 ELISA Kit,Cayman Chemical,Cat# No. 512031 Prostaglandin E2 ELISA Kit,Cayman Chemical,Cat# No. 514010 Prostaglandin E Metabolite ELISA Kit,Cayman Chemical,Cat# No. 514531 iScript TM cDNA Synthesis Kit,Bio-Rad,Cat#170–8891 eBioscience™

(12) Fever integrates antimicrobial defences, inflammation control, and tissue repair in a cold-blooded vertebrateeLifeMarch 14, 2023Farah Haddad, Amro M Soliman, Michael E Wong, Emilie H Albers, Shawna L Semple, Débora Torrealba, Ryan D Heimroth, Asif Nashiry, Keith B Tierney, Daniel R Barredasupernatant was collected, aliquoted, and stored at −80°C until use. Using a Prostaglandin E2 ELISA Kit (514010; Cayman Chemical, Ann Arbor, MI), plasma samples were diluted 1:30 and PGE2 protein concentrations were

(13) Appraisal of the Possible Role of PPARγ Upregulation by CLA of Probiotic Pediococcus pentosaceus GS4 in Colon Cancer MitigationPPAR ResearchFebruary 24, 2023Vinay Dubey, Alok Kumar Mishra, Asit Ranjan Ghoshapoptosis kit (K101) was procured from BioVision, Waltham, MA, USA. Prostaglandin E2 (PGE2) ELISA kit (514010), glucose uptake (600470), and Nicotinamide adenine dinucleotide/Nicotinamide adenine dinucleotide (Hydrogen)

(14) Bovine tumor necrosis factor-alpha Increases IL-6, IL-8, and PGE2 in bovine fibroblast-like synoviocytes by metabolic reprogrammingScientific ReportsFebruary 24, 2023Carolina Manosalva, Pablo Alarcon, John Quiroga, Stefanie Teuber, Maria D. Carretta, Hedie Bustamante, Rodrigo Lopez-Muñoz, Maria A. Hidalgo, Rafael A. BurgosSweden), IL-6 (#ESS0029, Thermo Fisher Scientific), IL-1β (#ESS0027, Thermo Fisher Scientific) and PGE2 (#514010, Cayman Chemicals, Ann Arbor, MI, USA) according to the manufacturer's instructions. Briefly, capture

(15) Attenuation of Lipopolysaccharide-Induced Inflammatory Responses through Inhibition of the NF-κB Pathway and the Increased NRF2 Level by a Flavonol-Enriched n-Butanol Fraction from Uvaria albaACS OmegaFebruary 14, 2023Kin Israel R. Notarte, Mark Tristan J. Quimque, Imee T. Macaranas, Abbas Khan, Adriel M. Pastrana, Oliver B. Villaflores, Hans Christian P. Arturo, Delfin Yñigo H. Pilapil IV, Sophia Morgan M. Tan, Dong-Qing Wei, et al.PGE2 concentration in the conditioned medium was measured using a commercial PGE2 ELISA kit (cat. no. 514010; Cayman Chemical Co., Ann Arbor, MI, USA) following the manufacturer’s instructions. 5.9 ELISA for

(16) Regulation of neuropathic pain by microglial Orai1 channelsScience advancesJanuary 27, 2023Tsujikawa S, DeMeulenaere KE, Centeno MV, Ghazisaeidi S, Martin ME, Tapies MR, Maneshi MM, Yamashita M, Stauderman KA, Apkarian AV, et al.(R&D Systems, catalog no. MTA00B), IL-6 (RayBiotech, catalog no. ELM-IL6-1), PGE2 (Cayman Chemical, catalog no. 514010), and MCP1 (RayBiotech, catalog no. ELM-MCP1-CL-5). All kits were used according to the manufacturers’

(17) Cell death-induced immunogenicity enhances chemoimmunotherapeutic response by converting immune-excluded into T-cell inflamed bladder tumorsNature CommunicationsDecember 1, 2022Fotis Nikolos et al.flow cytometry data were processed using FlowJo software v.10.7.1. ELISA ELISA analysis of PGE2 (Cayman, 514010) and HMGB1 (Arigo, ARG81310) released by cancer cells and serum from G69 and G7 tumor-bearing mice was

(18) Omega 3 supplementation reduces C-reactive protein, prostaglandin E2 and the granulocyte/lymphocyte ratio in heavy smokers: An open-label randomized crossover trialFrontiers in NutritionDecember 1, 2022Ingrid Elisia, Michelle Yeung, Sara Kowalski, Jennifer Wong, Hossein Rafiei, Roger A. Dyer, Sukhinder Atkar-Khattra, Stephen Lam, Gerald KrystalR&D Systems, Minneapolis, MN), LTB4 (cat # SKGE006B, R&D Systems, Minneapolis, MN) and PGE2 (cat # 514010, Cayman Chemical Company, Ann Arbor, MI) kits, according to the manufacturers' instructions. TGF-β (cat

(19) Tocilizumab overcomes chemotherapy resistance in mesenchymal stem-like breast cancer by negating autocrine IL-1A induction of IL-6npj Breast CancerDecember 1, 2022Andrew W. Chung, Anthony J. Kozielski, Wei Qian, Jianying Zhou, Ann C. Anselme, Alfred A. Chan, Ping-Ying Pan, Delphine J. Lee, Jenny C. ChangHuman prostaglandin E2 (PGE2) ELISA was performed according to the manufacturer’s instructions (Cayman 514010- Ann Arbor, MI). For in vivo analysis of human IL-6 in mice implanted with human cell lines, serum was

(20) The endocannabinoid anandamide is an airway relaxant in health and diseaseNat CommunNovember 17, 2022Annika Simon, Thomas von Einem, Alexander Seidinger, Michaela Matthey, Laura Bindila, Daniela WenzelThermo Fisher Scientific, USA). PGE2 was quantified in the supernatants using the Prostaglandin E2 ELISA Kit – Monoclonal (514010, Cayman Chemical, USA) and cAMP concentrations of tissue or cell lysates were determined with the Direct cAMP ELISA Kit

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Bench Tips for Optimizing ELISA: It All Comes Out in the Wash
August 22, 2013
This article will provide you with tips on washing your ELISA plates using an automated plate washer, with the goal of avoiding high background and high variation (between plates and between replicate wells). read more

Prostaglandin E2 ELISA Kit - Monoclonal from Cayman Chemical

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Cayman Chemical

Citations (187)

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Bench Tips for Optimizing ELISA: It All Comes Out in the Wash

Prostaglandin E2 ELISA Kit - Monoclonal from Cayman Chemical

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